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Overvågning intraspecies konkurrence i en bakteriel cellepopulation ved cocultivation af fluorescerende mærkede stammer
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JoVE Journal Biology
Monitoring Intraspecies Competition in a Bacterial Cell Population by Cocultivation of Fluorescently Labelled Strains

Overvågning intraspecies konkurrence i en bakteriel cellepopulation ved cocultivation af fluorescerende mærkede stammer

DOI:
10.3791/51196-v

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06:45 min

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January 18, 2014

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, , ,
1Department of General Microbiology,Georg-August University

Chapters

  • 00:05Title
  • 01:16Cocultivation of Bacteria, Sample Collection, and Sample Storage
  • 03:00Sample Treatment, Plating, and Incubation for Quantitative Analyses
  • 03:32Counting the Survivors by Stereo Fluorescence Microscopy for Quantitative Analysis
  • 04:46Results: Intraspecies Competition
  • 06:25Conclusion

Summary

Automatic Translation

Automatic Translation

Bakterier kan ophobes enten skadelige eller gavnlige mutationer i løbet af deres levetid. I en population af celler personer, der har akkumuleret gavnlige mutationer kan hurtigt udkonkurrere deres medmennesker. Her præsenterer vi en simpel procedure for at visualisere intraspecies konkurrence i en bakteriel cellepopulation over tid ved hjælp af fluorescerende mærkede individer.

Tags

Intraspecies CompetitionBacterial Cell PopulationFluorescently Labeled StrainsBacillus SubtilisClonal ExpansionBeneficial MutationsDetrimental MutationsSelectionGenotype ShiftPopulation DynamicsCocultivationMicrobial Competition

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