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神经干细胞和祖细胞在小鼠脑开发基因毒性应激后的评估细胞周期进程
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Assessing Cell Cycle Progression of Neural Stem and Progenitor Cells in the Mouse Developing Brain after Genotoxic Stress
DOI:

09:51 min

May 07, 2014

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Chapters

  • 00:05Title
  • 01:13Brain Slice Preparation
  • 03:03EdU and BrdU Staining
  • 06:05Imaging and Analysis
  • 06:59Identifying Apoptotic Cells and Cell Cycle Phase
  • 09:10Conclusion

Summary

Automatic Translation

胸苷两个类似物管理,埃杜和BrdU,在孕鼠允许在神经细胞和祖细胞在胚胎小鼠大脑细胞周期进程的分析。这个方法是有用的,以确定基因毒性应激,包括电离辐射,脑发育过程中的作用。

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