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JoVE Journal Neuroscience
Het beoordelen van de celcyclus van neurale stamcellen en stamcellen in de Mouse ontwikkeling van de hersenen na genotoxische stress
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Het beoordelen van de celcyclus van neurale stamcellen en stamcellen in de Mouse ontwikkeling van de hersenen na genotoxische stress

Article DOI: 10.3791/51209-v • 09:51 min • May 7th, 2014
May 7th, 2014 •
1,2,3,4, 1,2,3,4, 1,2,3,4, 1,2,3,4, 1,2,3,4
1Laboratoire de Radiopathologie, CEA DSV iRCM SCSR, 2INSERM, U967, 3Université Paris Diderot, Sorbonne Paris Cité, 4Université Paris Sud, UMR 967

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Toediening van twee analogen van thymidine, Edu en BrdU, bij zwangere muizen maakt de analyse van de celcyclus en in neurale voorlopercellen in de embryonale hersenen van muizen. Deze werkwijze is nuttig om de effecten van genotoxische stress, zoals ioniserende straling, tijdens de hersenontwikkeling bepalen.

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Neurowetenschappen Edu BrdU, neurale stamcellen en stamcellen celcyclus embryonale cortex immunostaining celcyclus apoptose genotoxische stress embronic hersenen van muizen
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