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Vurdering af cellecyklusprogression af neurale stam-og stamcelle i Mouse hjernens udvikling efter Genotoksisk Stress
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Assessing Cell Cycle Progression of Neural Stem and Progenitor Cells in the Mouse Developing Brain after Genotoxic Stress
DOI:

09:51 min

May 07, 2014

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Chapters

  • 00:05Title
  • 01:13Brain Slice Preparation
  • 03:03EdU and BrdU Staining
  • 06:05Imaging and Analysis
  • 06:59Identifying Apoptotic Cells and Cell Cycle Phase
  • 09:10Conclusion

Summary

Automatic Translation

Administration af to analoger af thymidin, Edu og BrdU, i gravide mus tillader analyse af cellecyklus progression i neurale og stamceller i embryonale mus hjernen. Denne metode er nyttig til at bestemme virkningerne af genotoksisk stress, herunder ioniserende stråling under hjernens udvikling.

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