Laser-induced Breakdown Spectroscopy: A New Approach for Nanoparticle's Mapping and Quantification in Organ Tissue

Lucie Sancey; Vincent Motto-Ros; Shady Kotb; Xiaochun Wang; Fran&#231;ois Lux; G&#233;rard Panczer; Jin Yu; Olivier Tillement

doi:10.3791/51353

Journal

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Engineering

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Laser-induced Breakdown Spectroscopy: A New Approach for Nanoparticle’s Mapping and Quantification in Organ Tissue

JoVE Journal
Engineering

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JoVE Journal Engineering

Laser-induced Breakdown Spectroscopy: A New Approach for Nanoparticle’s Mapping and Quantification in Organ Tissue

13,650 Views

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10:17 min

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June 18, 2014

DOI:

10.3791/51353-v

DOI:

10.3791/51353-v

•

10:17 min

June 18, 2014

•

3 Views

Lucie Sancey¹, Vincent Motto-Ros², Shady Kotb¹, Xiaochun Wang³, François Lux¹, Gérard Panczer³, Jin Yu², Olivier Tillement¹

¹ILM-FENNEC UMR 5306,CNRS – Université Lyon 1, ²ILM-PUBLI UMR 5306,CNRS – Université Lyon 1, ³ILM-SOPRANO UMR 5306,CNRS – Université Lyon 1

Summary

Laser-induced breakdown spectroscopy performed on thin organ and tumor tissue successfully detected natural elements and artificially injected gadolinium (Gd), issued from Gd-based nanoparticles. Images of chemical elements reached a resolution of 100 μm and quantitative sub-mM sensitivity. The compatibility of the setup with standard optical microscopy emphasizes its potential to provide multiple images of a same biological tissue.