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一种简单快速的协议用荧光测量中性脂肪在藻细胞
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Chemistry
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Chemistry
A Simple and Rapid Protocol for Measuring Neutral Lipids in Algal Cells Using Fluorescence
Please note that all translations are automatically generated.
Click here for the English version.
一种简单快速的协议用荧光测量中性脂肪在藻细胞
DOI:
10.3791/51441-v
•
10:56 min
•
May 30, 2014
•
Zachary J. Storms
,
Elliot Cameron
,
Hector de la Hoz Siegler
2
,
William C. McCaffrey
1
Department of Chemical and Materials Engineering
,
University of Alberta
,
2
Department of Chemical and Petroleum Engineering
,
University of Calgary
Chapters
00:05
Title
01:52
Isolation of Dry Algal Biomass to be Used as Standards for Fluorescence Readings
02:52
Gravimetric Quantification of Neutral Lipids by Hexane Extraction
04:48
Fluorometric Quantification of Neutral Lipids Using Nile Red
06:42
Fluorescence Microscopy Technique
08:04
Results: Images and Calibration Curves of
A. protothecoides
Stained with Nile Red
10:18
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
一个简单的协议采用尼罗红染色法来确定藻细胞中的中性脂质含量进行说明。这个节省时间的技术提供了一种替代传统的重量为基础的脂质定量协议。它被设计用于监测生物过程性能的具体应用。
Tags
Algal Cells
Neutral Lipids
Fluorescence
Nile Red
Auxenochlorella Protothecoides
Lipid Content
Bioprocess Monitoring
Rapid Protocol
Gravimetric Methods
Cell Membrane Permeabilization
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