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一种简单快速的协议用荧光测量中性脂肪在藻细胞
JoVE Journal
Chemistry
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JoVE Journal Chemistry
A Simple and Rapid Protocol for Measuring Neutral Lipids in Algal Cells Using Fluorescence
DOI:

10:56 min

May 30, 2014

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Chapters

  • 00:05Title
  • 01:52Isolation of Dry Algal Biomass to be Used as Standards for Fluorescence Readings
  • 02:52Gravimetric Quantification of Neutral Lipids by Hexane Extraction
  • 04:48Fluorometric Quantification of Neutral Lipids Using Nile Red
  • 06:42Fluorescence Microscopy Technique
  • 08:04Results: Images and Calibration Curves of A. protothecoides Stained with Nile Red
  • 10:18Conclusion

Summary

Automatic Translation

一个简单的协议采用尼罗红染色法来确定藻细胞中的中性脂质含量进行说明。这个节省时间的技术提供了一种替代传统的重量为基础的脂质定量协议。它被设计用于监测生物过程性能的具体应用。

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