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July 05, 2014
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Summary
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تنشيط بروتين للقناة الصوديوم الظهارية (عناق) أعرب heterologously في القيطم المورق يمكن البرهنة البويضات عن طريق الجمع بين القياسات الحالية مع نهج biotinylation للتحقيق في ظهور منتجات انشقاق القناة الايونية على سطح الخلية. ويمكن تحديد مواقع انشقاق مهم وظيفيا باستخدام الطفرات الموجهة الموقع.
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Cite this Article
Krappitz, M., Korbmacher, C., Haerteis, S. Demonstration of Proteolytic Activation of the Epithelial Sodium Channel (ENaC) by Combining Current Measurements with Detection of Cleavage Fragments. J. Vis. Exp. (89), e51582, doi:10.3791/51582 (2014).
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