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July 05, 2014
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Summary
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הפעלת מפרקי חלבונים של ערוץ אפיתל נתרן (ENaC) הביע heterologously בXenopus laevis ניתן להדגים ביציות על ידי שילוב של מדידות זרם עם גישת biotinylation לחקור את המראה של מוצרי מחשוף ערוץ יון על פני התא. ניתן לזהות אתרי מחשוף חשובים ופונקציונליים באמצעות מכוון mutagenesis אתר.
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Cite this Article
Krappitz, M., Korbmacher, C., Haerteis, S. Demonstration of Proteolytic Activation of the Epithelial Sodium Channel (ENaC) by Combining Current Measurements with Detection of Cleavage Fragments. J. Vis. Exp. (89), e51582, doi:10.3791/51582 (2014).
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