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July 05, 2014
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Activação proteolítica do canal de sódio epitelial (ENaC) heterologamente expressos em oócitos de Xenopus laevis pode ser demonstrada através da combinação de medições de corrente com uma abordagem de biotinilação para investigar o aparecimento de produtos de clivagem do canal de iões na superfície da célula. Locais de clivagem funcionalmente importantes podem ser identificados através de mutagénese dirigida ao local.
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Cite this Article
Krappitz, M., Korbmacher, C., Haerteis, S. Demonstration of Proteolytic Activation of the Epithelial Sodium Channel (ENaC) by Combining Current Measurements with Detection of Cleavage Fragments. J. Vis. Exp. (89), e51582, doi:10.3791/51582 (2014).
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