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上皮钠通道(ENaC)的蛋白水解激活通过结合电流测量与检测裂解片段示范
JoVE Journal
Biology
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JoVE Journal
Biology
Demonstration of Proteolytic Activation of the Epithelial Sodium Channel (ENaC) by Combining Current Measurements with Detection of Cleavage Fragments
Please note that all translations are automatically generated.
Click here for the English version.
上皮钠通道(ENaC)的蛋白水解激活通过结合电流测量与检测裂解片段示范
DOI:
10.3791/51582-v
•
08:56 min
•
July 05, 2014
•
Matteus Krappitz
,
Christoph Korbmacher
,
Silke Haerteis
1
Institut für Zelluläre und Molekulare Physiologie
,
Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU)
Chapters
00:05
Title
01:11
Two-electrode Voltage-clamp Experiments
02:41
Measurement of Amiloride-sensitive Whole-cell Currents
04:17
Biotinylation Assay and Detection of ENaC Cleavage Fragments at Cell Surface by Western Blot
07:08
Results: Proteolytic Activation of the Epithelial Sodium Channel
08:09
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
上皮钠通道(ENaC)的异源表达在
非洲爪蟾
的蛋白水解活化
蟾
卵母细胞可通过将当前测量与生物素化的方法,调查的离子通道的酶切产物在细胞表面的外观来证明。功能重要的切割位点可通过使用位点定向诱变来识别。
Tags
Proteolytic Activation
Epithelial Sodium Channel (ENaC)
Two-electrode Voltage-clamp
Amiloride-sensitive Current
Proteolytic Cleavage
Biotinylation
Gamma-ENaC Fragment
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