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上皮钠通道（ENaC）的蛋白水解激活通过结合电流测量与检测裂解片段示范
JoVE Journal
Biology
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JoVE Journal Biology
Demonstration of Proteolytic Activation of the Epithelial Sodium Channel (ENaC) by Combining Current Measurements with Detection of Cleavage Fragments
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上皮钠通道（ENaC）的蛋白水解激活通过结合电流测量与检测裂解片段示范

DOI:
10.3791/51582-v

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08:56 min

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July 05, 2014

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, ,
1Institut für Zelluläre und Molekulare Physiologie,Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU)

Chapters

  • 00:05Title
  • 01:11Two-electrode Voltage-clamp Experiments
  • 02:41Measurement of Amiloride-sensitive Whole-cell Currents
  • 04:17Biotinylation Assay and Detection of ENaC Cleavage Fragments at Cell Surface by Western Blot
  • 07:08Results: Proteolytic Activation of the Epithelial Sodium Channel
  • 08:09Conclusion

Summary

Automatic Translation

Automatic Translation

上皮钠通道（ENaC）的异源表达在非洲爪蟾的蛋白水解活化蟾卵母细胞可通过将当前测量与生物素化的方法，调查的离子通道的酶切产物在细胞表面的外观来证明。功能重要的切割位点可通过使用位点定向诱变来识别。

Tags

Proteolytic ActivationEpithelial Sodium Channel (ENaC)Two-electrode Voltage-clampAmiloride-sensitive CurrentProteolytic CleavageBiotinylationGamma-ENaC Fragment

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