12,790 Views
•
08:56 min
•
July 05, 2014
DOI:
Summary
Automatically generated
Proteolytische activering van het epitheliale natriumkanaal (ENaC) heteroloog tot expressie gebracht in Xenopus laevis oöcyten kan worden aangetoond door het combineren stroommetingen met een biotinylering benadering om het uiterlijk van ionkanaal splitsingsproducten op het celoppervlak te onderzoeken. Functioneel belangrijke knipplaatsen kunnen worden geïdentificeerd door plaatsgerichte mutagenese.
Related Videos
06:44
Demonstration of Cutaneous Allodynia in Association with Chronic Pelvic Pain
Related Videos
19570 Views
09:00
Detection of Protein Ubiquitination
Related Videos
42732 Views
09:30
Detection of Functional Matrix Metalloproteinases by Zymography
Related Videos
83622 Views
09:38
Analyzing the Function of Small GTPases by Microinjection of Plasmids into Polarized Epithelial Cells
Related Videos
11352 Views
07:42
Activation of Apoptosis by Cytoplasmic Microinjection of Cytochrome c
Related Videos
17316 Views
11:09
Chromatin Isolation by RNA Purification (ChIRP)
Related Videos
85953 Views
07:10
Agarose Gel Electrophoresis for the Separation of DNA Fragments
Related Videos
762326 Views
11:31
Single-molecule Imaging of Gene Regulation In vivo Using Cotranslational Activation by Cleavage (CoTrAC)
Related Videos
9803 Views
11:48
Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
Related Videos
12857 Views
10:24
Cytosolic Calcium Measurements in Renal Epithelial Cells by Flow Cytometry
Related Videos
15225 Views
Read Article
Cite this Article
Krappitz, M., Korbmacher, C., Haerteis, S. Demonstration of Proteolytic Activation of the Epithelial Sodium Channel (ENaC) by Combining Current Measurements with Detection of Cleavage Fragments. J. Vis. Exp. (89), e51582, doi:10.3791/51582 (2014).
Copy