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人类iPS细胞的微小RNA表达谱，视网膜色素上皮衍生自IPS和胎儿视网膜色素上皮
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JoVE Journal Biology
MicroRNA Expression Profiles of Human iPS Cells, Retinal Pigment Epithelium Derived From iPS, and Fetal Retinal Pigment Epithelium
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人类iPS细胞的微小RNA表达谱，视网膜色素上皮衍生自IPS和胎儿视网膜色素上皮

DOI:
10.3791/51589-v

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10:19 min

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June 24, 2014

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, , , ,
1Ocular Trauma, U.S. Army Institute of Surgical Research,JBSA Fort Sam Houston

Chapters

  • 00:05Title
  • 01:41Differentiation of iPS Cells to iPS-Retinal Pigmented Epithelium
  • 03:32Collection of Cell Samples
  • 05:15Removal of Undifferentiated Cells from iPS-RPE Cells by Negative Selection
  • 06:36Total RNA Extraction
  • 07:08Pathway Analysis
  • 08:20Results: Differences in miRNA Expression Profiles of iPS-RPE as Compared to iPS and Fetal RPE
  • 09:21Conclusion

Summary

Automatic Translation

Automatic Translation

微小RNA（miRNA）的由人诱导多能干细胞（iPS）细胞（iPS-RPE）和胎儿视网膜色素上皮衍生的人诱导多能干细胞的访问（iPS细胞），视网膜色素上皮细胞（RPE），进行比较。

Tags

MicroRNAMiRNAIPS CellsRetinal Pigment EpitheliumRPEFetal RPERNA ExtractionMicroarrayCellular PathwaysNetwork Analysis

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