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冷冻断裂/冷冻蚀刻的生物电子显微技术的基本要素
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Biology
Fundamental Technical Elements of Freeze-fracture/Freeze-etch in Biological Electron Microscopy
Please note that all translations are automatically generated.
Click here for the English version.
冷冻断裂/冷冻蚀刻的生物电子显微技术的基本要素
DOI:
10.3791/51694-v
•
11:17 min
•
September 11, 2014
•
Johnny L. Carson
1
Department of Pediatrics, Center for Environmental Medicine, Asthma, and Lung Biology
,
The University of North Carolina at Chapel Hill
Chapters
00:05
Title
02:15
Freezing and Storage of Specimens in Advance of Freeze-fracture
04:43
Operation of the Freeze-fracture Instrument and Specimen Processing
08:45
Results: Structures Revealed by Freeze-fracture
10:28
Conclusion
Summary
Automatic Translation
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基本的技术和用于检查通过透射电子显微镜生物标本和纳米材料的冷冻断裂加工的精炼进行说明。这种技术对于揭示超微结构特点和生物膜的专业和获得超微结构水平尺寸和空间数据在材料科学和纳米技术产品的首选方法。
Tags
Freeze-fracture
Freeze-etch
Electron Microscopy
Biological Specimens
Cell Membranes
Ultrarapid Freezing
Cryoprotective Agents
Fracturing
Carbon-platinum Replication
Three-dimensional Detail
Cell Ultrastructure
Cell Biology
Nanotechnology
Materials Science
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