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Whole-cell Recordings patch-clamp de Morphologically- e identificados Neuroquimicamente hipocampo Interneurônios
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Chapters
- 00:05Title
- 01:46Preparation of Acute-hippocampal Slices
- 03:00Whole Cell Patch-clamp Recording from Fast-spiking Interneurons
- 05:58Extracellular Electrical Stimulation to Evoke GABAB Receptor-mediated Responses
- 06:56Paired Recordings of Synaptically Coupled Fast-spiking INs and CA1 PCs
- 08:42Visualization and Immunocytochemistry of Fast-spiking Interneurons
- 11:30Results: GABAB Receptor-mediated Currents and Presynapitc Inhibition in Identified Hippocampal Parvalbumin Interneurons
- 13:59Conclusion
Redes corticais são controlados por um conjunto de pequenos, mas diversificada de interneurónios inibitórios. Investigação funcional dos interneurônios, portanto, requer a gravação específica e rigorosa identificação. Descrito aqui é uma abordagem conjunta, envolvendo gravações de células inteiras de pares simples ou synaptically acoplados de neurônios com rotulagem intracelular, post-hoc Análise morfológica e imunocitoquímica.
Tags
Keywords: Whole-cell Patch-clampHippocampal InterneuronsGABAergic Inhibitory InterneuronsNeuronal CircuitsPhysiological HeterogeneityMorphological HeterogeneityNeurochemical HeterogeneityTransgenic AnimalsFluorescent ProteinsIntrinsic PropertiesSynaptic PropertiesIntracellular Dye LabelingMorphological AnalysisImmunocytochemical Analysis
