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Generation of Human cardiomyocytes: En Differentiering Protocol fra Feeder-fri Menneskelige induceret pluripotente stamceller
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JoVE Journal Biology
Generation of Human Cardiomyocytes: A Differentiation Protocol from Feeder-free Human Induced Pluripotent Stem Cells

Generation of Human cardiomyocytes: En Differentiering Protocol fra Feeder-fri Menneskelige induceret pluripotente stamceller

DOI:
10.3791/50429-v

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13:18 min

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June 28, 2013

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, ,
1Humanitas Clinical and Research Center, Italy, 2Institute of Genetic and Biomedical Research (IRGB),National Research Council (CNR)

Chapters

  • 00:05Title
  • 02:15Feeder-free Maintenance and Passaging of Human iPS Cell Lines
  • 03:54Embryoid Bodies Aggregation and Cardiac Induction
  • 06:25Beating Areas Isolation and Culture
  • 07:48Single Beating Cells Isolation and Analysis
  • 10:06Characterizing Contracting Cardiomyocytes Derived from iPS Cells
  • 12:36Conclusion

Summary

Automatic Translation

Automatic Translation

Pluripotente stamceller, enten embryonale eller induceret pluripotente stamceller (iPS-celler), udgør en værdifuld kilde til humane differentierede celler, herunder cardiomyocytter. Her vil vi fokusere på hjertets induktion af iPS celler, der viser, hvordan man bruger dem til at opnå funktionelle menneskelige cardiomyocytes gennem en embryoid organer-baseret protokol.

Tags

Keywords: Human CardiomyocytesInduced Pluripotent Stem CellsDifferentiation ProtocolFeeder-freeHeart DevelopmentMyocardial DiseasesDrug DiscoveryToxicologyCardiac DisordersEmbryoid BodiesChemically Defined Protocols

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