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Måling af mitokondriel masse og membran potentiale i hæmatopoietiske stamceller og T-celler ved flow cytometri
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JoVE Journal Biology
Measurement of Mitochondrial Mass and Membrane Potential in Hematopoietic Stem Cells and T-cells by Flow Cytometry

Måling af mitokondriel masse og membran potentiale i hæmatopoietiske stamceller og T-celler ved flow cytometri

DOI:
10.3791/60475-v

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07:57 min

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December 26, 2019

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, , , , ,
1Department of Oncology UNIL CHUV, Ludwig Institute for Cancer Research Lausanne,University of Lausanne, 2Swiss Institute for Experimental Cancer Research (ISREC), School of Life Sciences,Swiss Federal Institute of Technology Lausanne (EPFL), 3Center of Experimental Therapeutics, Department of Oncology,Centre Hospitalier Universitaire Vaudois, 4Hematology Service, Department of Oncology,Centre Hospitalier Universitaire Vaudois (CHUV)

Chapters

  • 00:04Title
  • 01:59Ex Vivo Culture of FACS Sorted Hematopoietic Stem Cells
  • 04:00Measurement of Mitochondrial Mass and Membrane Potential
  • 06:21Results: Mitochondrial Profiles
  • 07:18Conclusion

Summary

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Her beskriver vi en pålidelig metode til måling af mitokondriel masse og membran potentiale i ex vivo kulturperler hæmatopoietiske stamceller og T-celler.

Tags

Mitochondrial MassMitochondrial Membrane PotentialFlow CytometryHematopoietic Stem CellsT-cellsMetabolic AssaysBone Marrow TransplantationColony-forming AssaysImmune T CellsVerapamilFACS SortingLKS PopulationCD150CD48Stem Cell Expansion Medium

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