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Chapters
- 00:05Title
- 02:08Preparation of Spontaneously Formed Vesicle (SFV) Dispersion
- 03:54Extrusion and Purification of Homogeneous Liposome Vesicles Encapsulating DY-676-COOH
- 05:21Physico-chemical Analysis of Flourescence Quenching and Activation
- 07:10Induction of Inflammation and In Vivo NIRF Imaging
- 08:10Results: Representative Liposomal Flourescence Quenching Analyses
- 09:54Conclusion
The use of fluorophores for in vivo imaging can be greatly limited by opsonization, rapid clearance, low detection sensitivity and cytotoxic effects on the host. Encapsulation of fluorophores in liposomes by film hydration and extrusion leads to fluorescence quenching and protection which enables in vivo imaging with high detection sensitivity.
