A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Department of Pathology,Memorial Sloan Kettering Cancer Center, 2Molecular Pharmacology and Chemistry Program,Memorial Sloan Kettering Cancer Center, 3Department of Radiology,Weill Cornell Medical College, 4Department of Medicine,Memorial Sloan Kettering Cancer Center, 5Department of Oncology,Memorial Sloan Kettering Cancer Center, 6Department of Surgery,Memorial Sloan Kettering Cancer Center
Chapters
- 00:05Title
- 02:30Tissue Procurement
- 04:09Preparation of Materials for Sectioning and Vibratome Settings
- 05:19Tissue Sectioning, Treatment, and Analyses
- 06:25Results: Ex Vivo Metastatic Breast Cancer Response to Heat Shock Protein 90 Inhibitor
- 07:56Conclusion
Linhas celulares de cancro estabelecidos e xenotransplantes têm sido o esteio da pesquisa do câncer para as últimas décadas. No entanto, evidências recentes sugerem que a resposta terapêutica é muito influenciada pelo microambiente de células tumorais. Portanto, foi desenvolvida uma análise ex vivo de amostras de tumor primário para fins de desenvolvimento de drogas.
