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1Egg Safety and Quality Research Unit,USDA-Agricultural Research Service, 2Poultry Microbiological Safety and Processing Research Unit,USDA-Agricultural Research Service, 3Department of Biochemistry and Biophysics,Oregon State University, 4College of Public Health,University of Georgia, 5Department of Biological Sciences, Center for Microbial Genetics and Genomics,Northern Arizona University
Chapters
- 00:05Title
- 01:56Mechanical Homogenization
- 03:32Enzymatic Inhibition of Inhibitors from Sample Homogenates
- 04:51Automated DNA Purification
- 06:25Results: Hybrid DNA Extraction Method Effective for Total Bacterial Assessment
- 06:54Conclusion
A novel semi-automated hybrid DNA extraction method for use with environmental poultry production samples was developed and demonstrated improvements over a common mechanical and enzymatic extraction method in terms of the quantitative and qualitative estimates of the total bacterial communities.
