Journal
/
Immunology and Infection
/
Modeling Mucosal Candidiasis in Larval Zebrafish by Swimbladder Injection
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Modeling Mucosal Candidiasis in Larval Zebrafish by Swimbladder Injection

Modeling Mucosal Candidiasis in Larval Zebrafish by Swimbladder Injection

DOI:
10.3791/52182-v

•

06:28 min

•

November 27, 2014

•

, ,
1Department of Molecular and Biomedical Sciences,University of Maine, 2Graduate School of Biomedical Sciences and Engineering,University of Maine

Chapters

  • 00:05Title
  • 01:14Injecting Zabrafish in the Swimbladder
  • 03:11Dissecting the Swimbladder
  • 04:21Results: Representative C. albicans–infected Zebrafish Imaging
  • 05:59Conclusion

Summary

Automatic Translation

Automatic Translation

In vivo spatio-temporal interactions of pathogen and immune defenses at the mucosal level are not easily imaged in existing vertebrate hosts. The method presented here describes a versatile platform to study mucosal candidiasis in live vertebrates using the swimbladder of the juvenile zebrafish as an infection site.

Tags

Keywords: Mucosal CandidiasisLarval ZebrafishSwimbladder InjectionEpithelial BarrierInnate Immune CellsCandida AlbicansHost-pathogen InteractionMucosal Infection ModelPhagocyte RecruitmentMucosal Pathogens

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/52182?article_type=v

Non-invasive Imaging of Disseminated Candidiasis in Zebrafish Larvae
check_url/52182?article_type=v

In vitro Coculture Assay to Assess Pathogen Induced Neutrophil Trans-epithelial Migration
check_url/52182?article_type=v

Non-invasive Imaging of the Innate Immune Response in a Zebrafish Larval Model of Streptococcus iniae Infection
check_url/52182?article_type=v

Noninvasive Sampling of Mucosal Lining Fluid for the Quantification of In Vivo Upper Airway Immune-mediator Levels
check_url/52182?article_type=v

Bile Salt-induced Biofilm Formation in Enteric Pathogens: Techniques for Identification and Quantification
check_url/52182?article_type=v

Modeling Tuberculosis in Mycobacterium marinum Infected Adult Zebrafish
check_url/52182?article_type=v

The Galleria mellonella Waxworm Infection Model for Disseminated Candidiasis
check_url/52182?article_type=v

Induction of Mouse Lung Injury by Endotracheal Injection of Bleomycin
check_url/52182?article_type=v

Targeting Drugs to Larval Zebrafish Macrophages by Injecting Drug-Loaded Liposomes
check_url/52182?article_type=v

Isolation of Tonsillar Mononuclear Cells to Study Ex Vivo Innate Immune Responses in a Human Mucosal Lymphoid Tissue

Read Article