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器官切片文化为产后神经再生的研究
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Developmental Biology
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JoVE Journal Developmental Biology
Organotypic Slice Cultures for Studies of Postnatal Neurogenesis
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器官切片文化为产后神经再生的研究

DOI:
10.3791/52353-v

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08:52 min

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March 04, 2015

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, , ,
1Department of Physiology,University of Toronto, 2Institute of Anatomy and Cell Biology, School of Medicine,National Yang-Ming University, 3Department of Education and Research,Taipei City Hospital

Chapters

  • 00:05Title
  • 01:21Hippocampal Dissection
  • 04:13Feeding and Maintaining Organotypic Slices
  • 04:47Labelling, Fixation and Storage
  • 05:44Sectioning Tissue for Immunohistochemistry
  • 07:30Results: Postnatal Neurogenesis
  • 08:24Conclusion

Summary

Automatic Translation

Automatic Translation

在这里，我们描述了学习的海马神经发生产后使用的器官切片培养技术的技术。此方法允许在体外操纵成年神经发生，并允许直接应用的药剂的培养海马。

Tags

Organotypic Slice CulturePostnatal NeurogenesisHippocampusDentate GyrusPharmacological ManipulationDeveloping Granule NeuronsMaturation ProcessRodent Brain

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