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JoVE Journal

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Essential Metal Uptake in Gram-negative Bacteria

Essential Metal Uptake in Gram-negative Bacteria: X-ray Fluorescence, Radioisotopes, and Cell Fractionation

Article DOI: 10.3791/57169
February 1st, 2018


Summary February 1st, 2018

A protocol for the extraction of a periplasmic transition metal chaperone in the context of its native binding partners, and biophysical characterization of its substrate contents by X-ray fluorescence and radiometal uptake is presented.


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