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Détermination Membrane Protein topologie utilisant la fluorescence protection protéase (FPP)
Determining Membrane Protein Topology Using Fluorescence Protease Protection (FPP)
JoVE Journal
Biology
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JoVE Journal Biology
Determining Membrane Protein Topology Using Fluorescence Protease Protection (FPP)

Détermination Membrane Protein topologie utilisant la fluorescence protection protéase (FPP)

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17,777 Views

08:14 min

April 20, 2015

DOI:

08:14 min
April 20, 2015

17760 Views
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Summary

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Here, we present a protocol to determine the orientation and topology of integral membrane proteins in living cells. This simple protocol relies on selective protease sensitivity of chimeras between the protein of interest and GFP.

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