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April 20, 2015
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Here, we present a protocol to determine the orientation and topology of integral membrane proteins in living cells. This simple protocol relies on selective protease sensitivity of chimeras between the protein of interest and GFP.
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Cite this Article
White, C., Nixon, A., Bradbury, N. A. Determining Membrane Protein Topology Using Fluorescence Protease Protection (FPP). J. Vis. Exp. (98), e52509, doi:10.3791/52509 (2015).
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