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Chapters
- 00:05Title
- 01:25Endothelialization of Multi-organ Co-culture
- 04:37Loading the Chip
- 05:32Connecting the Chip to the Pump Control Unit
- 06:24On-line Analysis of Medium Samples
- 07:51Results: Multi-tissue Co-culture Using the Multi-organ Chip
- 09:36Conclusion
Here, we present a protocol to coculture primary cells, tissue models and punch biopsies in a microfluidic multi-organ chip for up to 28 days. Human dermal microvascular endothelial cells, liver aggregates and skin biopsies were successfully combined in a common media circulation.
