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表面增强拉曼光谱检测生物分子利用EBL装配纳米衬底
JoVE Journal
Engineering
This content is Free Access.
JoVE Journal Engineering
Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
DOI:

11:44 min

March 20, 2015

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Chapters

  • 00:05Title
  • 02:04Fabrication of Nano-patterned PMMA Masks Using Electron Beam Lithography (EBL)
  • 03:47Noble Metal Nanostructures Fabrication
  • 04:41Bio-functionalization of Substrates
  • 08:14Raman Spectroscopy
  • 09:09Results: Nanostructure-enabled SERS Spectra of Biopolymers
  • 10:29Conclusion

Summary

Automatic Translation

We describe the fabrication and characterization of nano-biological systems interfacing nanostructured substrates with immobilized proteins and aptamers. The relevant experimental steps involving lithographic fabrication of nanostructured substrates, bio-functionalization, and surface-enhanced Raman spectroscopy (SERS) characterization, are reported. SERS detection of surface-immobilized proteins, and probing of protein-ligand and aptamer-ligand binding is demonstrated.

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