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胞浆的定量与液泡<em>沙门氏菌</em>在原发性巨噬细胞差透
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Immunology and Infection
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JoVE Journal Immunology and Infection
Quantification of Cytosolic vs. Vacuolar Salmonella in Primary Macrophages by Differential Permeabilization
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胞浆的定量与液泡<em>沙门氏菌</em>在原发性巨噬细胞差透

DOI:
10.3791/52960-v

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09:25 min

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July 28, 2015

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,
1Focal Area Infection Biology, Biozentrum,University of Basel

Chapters

  • 00:05Title
  • 01:02Preparation for Digitonin Assay with mCherry-expressing Salmonella
  • 04:59Infected Cells: FACS Analysis
  • 06:03Uninfected Permeabilization Controls: Microscopy
  • 07:15Results: Quantification of Cytosolic Salmonella Typhimurium
  • 08:59Conclusion

Summary

Automatic Translation

Automatic Translation

We describe the quantification of cytosolic and vacuolar Salmonella typhimurium in bone-marrow derived macrophages using differential digitonin permeabilization.

Tags

SalmonellaIntracellular BacteriaCytosolVacuoleSalmonella-containing Vacuole (SCV)Differential PermeabilizationPhagosome-protection AssayDigitoninFluorescence MicroscopyFlow Cytometry

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