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Chapters
- 00:05Title
- 01:37Plate Seeding with a Manual Multiple-channel Pipetter
- 02:33Plate Seeding with a Robotic Pipetter
- 05:23Plate Drugging Using a Robotic Pipettor and Seeded Cell Imaging
- 07:36Results: Representative Robotic Seeding and Drug Treatment of MM Cells
- 09:01Conclusion
We describe a high-throughput drug sensitivity assay for primary multiple myeloma cells. It consists of a reconstruction of the bone marrow microenvironment (including extracellular matrix and stroma) in multi-well plates, and a non-invasive method for longitudinal quantification of cell viability.
