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Neuroscience
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正常小鼠β淀粉样肽侧脑室注射敏锐地诱导老年痴呆样认知缺陷
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JoVE Journal
Neuroscience
Intracerebroventricular Injection of Amyloid-β Peptides in Normal Mice to Acutely Induce Alzheimer-like Cognitive Deficits
Please note that all translations are automatically generated.
Click here for the English version.
正常小鼠β淀粉样肽侧脑室注射敏锐地诱导老年痴呆样认知缺陷
DOI:
10.3791/53308-v
•
08:01 min
•
March 16, 2016
•
Hye Yun Kim
2
,
Dongkeun K. Lee
,
Bo-Ryehn Chung
3
,
Hyunjin V. Kim
4
,
YoungSoo Kim
4
1
Center for Neuro-Medicine
,
Korea Institute of Science and Technology
,
2
Research Institute
,
GoshenBiotech, Inc.
,
3
Department of Chemical and Biological Engineering
,
Princeton University
,
4
Biological Chemistry Program
,
Korea University of Science and Technology
Chapters
00:05
Title
01:30
Aβ Peptide and Syringe Preparation
02:54
Aβ Injection in Mouse
06:06
Results: Confirmation of Prepared Aβ Species via SDS-PAGE and Y-maze Behavioral Tests
07:07
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
淀粉样蛋白β(Aβ)-injected动物模型能够确定的数量和Aβ片段的物种的管理,并降低每个研究组内的个体差异。本协议描述Aβ的脑室(ICV)注射立体定向仪,使生产正常小鼠老年痴呆样行为异常。
Tags
Intracerebroventricular Injection
Amyloid-beta Peptides
Alzheimer’s Disease
Cognitive Deficits
Mouse Model
Stereotactic Instruments
Drug Discovery
Behavior Abnormalities
Bregma
DMSO
PBS
Microsyringe
26-gauge Needle
Parafilm
Ethanol
UV Light
Anesthesia
Toe Pinch Reflex
Mirrors
Perpendicular Injection
Article
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