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Enrichissement de la membrane de Bruch des yeux de donneur humain
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Enrichment of Bruch’s Membrane from Human Donor Eyes
DOI:

10:22 min

November 15, 2015

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Chapters

  • 00:05Title
  • 01:14Enrichment of Whole Bruch’s Membrane
  • 05:00Solubilization of Whole Enriched Bruch’s Membrane and Western Blot Analysis
  • 06:34Enrichment of Macular Bruch’s Membrane
  • 08:51Results: Histology and Protein Content of Bruch’s Membrane
  • 09:35Conclusion

Summary

Automatic Translation

Identifying proteins specifically associated with Bruch’s membrane in human eyes is an important step in understanding the biochemical mechanisms behind eye diseases such as age-related macular degeneration. This protocol describes how to enrich this sheet of extracellular matrix for down-stream biochemical analysis.

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