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Developmental Biology

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JoVE Journal Developmental Biology
Culture ex vivo de Chick cervelet tranches et spatialement ciblées électroporation de granules précurseurs de cellules
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Journal (Developmental Biology)
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Precise Cellular Ablation Approach for Modeling Acute Kidney Injury in Developing Zebrafish
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Culture ex vivo de Chick cervelet tranches et spatialement ciblées électroporation de granules précurseurs de cellules

Article DOI: 10.3791/53421
December 14th, 2015 •
1, 1, 2
1MRC Centre of Developmental Neurobiology, King's College London, 2School of Biological and Chemical Sciences, Queen Mary, University of London

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December 14th, 2015

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La couche de granules cérébelleux externe est le site de la plus grande amplification de transit dans le cerveau en développement. Ici, nous présentons un protocole de cibler la modification génétique de cette couche au sommet de la prolifération utilisant ex électroporation et la culture de tranches de cervelet vivo à partir embryonnaires Jour 14 embryons de poulet.

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