A protocol is described for the preparation of high-quality mitotic plant chromosome spreads by a fast air-dry dropping method suitable for the FISH detection of single and high copy DNA probes.
Aliyeva-Schnorr, L., Ma, L., Houben, A. A Fast Air-dry Dropping Chromosome Preparation Method Suitable for FISH in Plants. J. Vis. Exp. (106), e53470, doi:10.3791/53470 (2015).