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全山解剖和成年小鼠耳蜗的免疫
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Whole Mount Dissection and Immunofluorescence of the Adult Mouse Cochlea
DOI:

12:02 min

January 01, 2016

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Chapters

  • 00:05Title
  • 00:59Preparation of Temporal Bones
  • 02:14Whole Mount Dissection of the Cochlea
  • 05:41Immunostaining with Fluorescently Tagged Antibodies
  • 07:30Mounting Stained Cochlear Turns
  • 08:59Results: Examples of Optimal and Suboptimal Dissection and Immunostaining
  • 10:54Conclusion

Summary

Automatic Translation

我们提出了一个方法,以分离科尔蒂的成年器官三个完整耳蜗匝(先端,中间,和碱)。我们还演示了一个程序,用荧光标记的抗体免疫染色。连同这些技术允许毛细胞,支持细胞,以及其他细胞类型中的耳蜗研究发现。

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