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Neuroscience
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全山解剖和成年小鼠耳蜗的免疫
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Whole Mount Dissection and Immunofluorescence of the Adult Mouse Cochlea
Please note that all translations are automatically generated.
Click here for the English version.
全山解剖和成年小鼠耳蜗的免疫
DOI:
10.3791/53561-v
•
12:02 min
•
January 01, 2016
•
Scott C. Montgomery
,
Brandon C. Cox
2
1
Department of Surgery, Division of Otolaryngology
,
Southern Illinois University, School of Medicine
,
2
Department of Pharmacology
,
Southern Illinois University, School of Medicine
Chapters
00:05
Title
00:59
Preparation of Temporal Bones
02:14
Whole Mount Dissection of the Cochlea
05:41
Immunostaining with Fluorescently Tagged Antibodies
07:30
Mounting Stained Cochlear Turns
08:59
Results: Examples of Optimal and Suboptimal Dissection and Immunostaining
10:54
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们提出了一个方法,以分离科尔蒂的成年器官三个完整耳蜗匝(先端,中间,和碱)。我们还演示了一个程序,用荧光标记的抗体免疫染色。连同这些技术允许毛细胞,支持细胞,以及其他细胞类型中的耳蜗研究发现。
Tags
Cochlea
Whole Mount Dissection
Immunofluorescence
Organ Of Corti
Temporal Bone
Otic Capsule
Spiral Ligament
Decalcification
Forceps
Vannas-Tubingen Scissors
Confocal Microscopy
Z-axis Imaging
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