Polygraphic Recording Procedure for Measuring Sleep in Mice

The overall goal of this polygraphic recording procedure is to determine the vigilant state of mice and measure the time spent in wakefulness, rapid eye movement sleep or non-rapid eye movement sleep. This method can help answer key questions in nearly every neuroscience field that seeks to correlate behavior and physiology with the activity of cortical neurons in behaving animals. The main advantage of this technique is that it can record a lot of nodes, electroencephalogram and electromyogram and can be combined with other brain implants such as optic fibers and infusion cannulas.

Visual demonstration of this method is very important because the implantation of the electrode needs to be performed properly and the quality of the electrode is extremely critical to judge the physical states. Demonstrating the procedure will be Yohko Takata, a postdoc from my laboratory. Before starting, sterilize all the surgical tools in a hot bead sterilizer.