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Journal / Chemistry / Immunfärbung Phospho-Epitope in Ciliated Organe…
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JoVE Journal Chemistry
Immunfärbung Phospho-Epitope in Ciliated Organe Ganze Berge Zebrafischembryonen
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Immunfärbung Phospho-Epitope in Ciliated Organe Ganze Berge Zebrafischembryonen

Article DOI: 10.3791/53747-v • 08:42 min • February 19th, 2016
February 19th, 2016 •
1, 2, 1
1Department of Biology, Virginia Commonwealth University, 2Center for Human Disease Modeling, Duke University Medical Center

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Es sind Techniken beschrieben Phospho-Epitope in ganz Zebrafischembryonen zu Immunfärbung und dann leiten zweifarbige Leuchtstoff konfokalen Lokalisierung in zellularen Strukturen so klein wie primäre Zilien. Die Techniken zur Fixierung und Bildgebung können die Position und die Kinetik des Erscheinens oder Aktivierung spezifischer Proteine ​​definieren.

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Chemie Heft 108 Fixierung Phospho-Epitope Kalzium Zilien Mikroskopie Kupffer der Vesikel Niere Ohr Zebrafisch
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