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JoVE Journal Developmental Biology
Feeder-fri Udledning af melanocytter fra menneskelige pluripotente stamceller
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Feeder-fri Udledning af melanocytter fra menneskelige pluripotente stamceller

Article DOI: 10.3791/53806 • 12:21 min • March 3rd, 2016
March 3rd, 2016 •
1,2, 3, 1
1The Center for Stem Cell Biology, Developmental Biology Program, Memorial Sloan-Kettering Cancer Center, 2Cancer Biology and Genetics Program, Gerstner Sloan-Kettering Graduate School, Sloan-Kettering Institute for Cancer Research, 3Thermo Fisher Scientific

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Dette arbejde beskriver en in vitro differentiering protokol til at producere pigmenterede, modne melanocytter fra humane pluripotente stamceller via en neural crest og melanoblast mellemtrin ved hjælp af en feeder-fri, 25 dage protokol.

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Developmental Biology melanocytter pigmentering melanoblast embryonale stamceller (EKSF) menneskelige pluripotente stamceller (hPSCs) induceret pluripotente stamceller (iPSCs) Neural Crest in vitro differentiering sygdom modellering differentiering protokol menneskelige embryonale stamceller
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