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Differentierende chondrocytter fra perifert blodafledte humane inducerede pluripotente stamceller
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Developmental Biology
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JoVE Journal Developmental Biology
Differentiating Chondrocytes from Peripheral Blood-derived Human Induced Pluripotent Stem Cells

Differentierende chondrocytter fra perifert blodafledte humane inducerede pluripotente stamceller

DOI:
10.3791/55722-v

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07:51 min

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July 18, 2017

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, , ,
1Department of Orthopedics, Beijing Chao-Yang Hospital,Capital Medical University, 2Key Laboratory of Genomic and Precision Medicine, Beijing Institute of Genomics,Chinese Academy of Sciences, 3Clinical and Translational Research Center of Shanghai First Maternity & Infant Hospital, School of Life Sciences and Technology,Tongji University

Chapters

  • 00:05Title
  • 00:47Embryoid Body (EB) Formation
  • 03:37Cell Pellet Formation and Chondrocyte Differentiation
  • 05:42Results: Representative hiPSC-Chon Pellet Characterization
  • 06:54Conclusion

Summary

Automatic Translation

Automatic Translation

Vi præsenterer en protokol for at generere en chondrogen afstamning fra humant perifert blod (PB) via inducerede pluripotente stamceller (iPSCs) ved hjælp af en integrationsfri metode, der omfatter embryoidlegem (EB) dannelse, ekspansion af fibroblast celler og chondrogen induktion.

Tags

Keywords: ChondrocytesPeripheral BloodInduced Pluripotent Stem CellsIPS CellsCartilage RegenerationChondrogenic DifferentiationSerum-freeXeno-freeEmbryoid BodiesFibroblastic CellsGelatinBasal Culture Medium

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