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Effektiv differentiering af postganglioniske sympatiske neuroner ved hjælp af humane pluripotente stamceller under Feeder-fri og kemisk definerede kulturbetingelser
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Developmental Biology
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JoVE Journal Developmental Biology
Efficient Differentiation of Postganglionic Sympathetic Neurons using Human Pluripotent Stem Cells under Feeder-free and Chemically Defined Culture Conditions

Effektiv differentiering af postganglioniske sympatiske neuroner ved hjælp af humane pluripotente stamceller under Feeder-fri og kemisk definerede kulturbetingelser

English

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14,005 Views

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10:24 min

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May 24, 2020

DOI:

10.3791/60843-v

DOI:
10.3791/60843-v

•

10:24 min
May 24, 2020

•

32 Views
,
1Center for Molecular Medicine,University of Georgia, 2Department of Biochemistry and Molecular Biology, Franklin College of Arts and Sciences,University of Georgia, 3Department of Cellular Biology, Franklin College of Arts and Sciences,University of Georgia

Summary

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I denne protokol beskriver vi en stabil, yderst effektiv differentieringsstrategi for generering af postganglioniske sympatiske neuroner fra menneskelige pluripotente stamceller. Denne model vil gøre neuroner til rådighed for brug af undersøgelser af flere autonome lidelser.

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Cite this Article

Wu, H. F., Zeltner, N. Efficient Differentiation of Postganglionic Sympathetic Neurons using Human Pluripotent Stem Cells under Feeder-free and Chemically Defined Culture Conditions. J. Vis. Exp. (159), e60843, doi:10.3791/60843 (2020).

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