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Journal / Developmental Biology / In vitro neuromuskulær junction induceret fra…
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Developmental Biology

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JoVE Journal Developmental Biology
In vitro neuromuskulær junction induceret fra humane inducerede pluripotente stamceller
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In vitro neuromuskulær junction induceret fra humane inducerede pluripotente stamceller

Article DOI: 10.3791/61396-v • 06:01 min • December 3rd, 2020
December 3rd, 2020 •
1, 1,2, 3, 1
1Department of Clinical Application, Center for iPS Cell Research and Application (CiRA), Kyoto University, 2Department of Pediatrics, Kyoto Prefectural University of Medicine, 3Graduate Institute of Clinical Medicine, Taipei Medical University

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Her leverer vi en protokol til at generere in vitro NMJs fra humane inducerede pluripotente stamceller (iPSCs). Denne metode kan fremkalde NMJs med modne morfologi og funktion i 1 måned i en enkelt brønd. De resulterende NMJs kunne potentielt bruges til at modellere relaterede sygdomme, til at studere patologiske mekanismer eller til at screene lægemiddelforbindelser til behandling.

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Udviklingsmæssige Biologi iPSCs in vitro neuromuskulære kryds motor neuron Schwann celle myotube sammentrækning curare
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