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Immunology and Infection

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JoVE Journal Immunology and Infection
Visualization of Twitching Motility and Characterization of the Role of the PilG in Xylella fastidiosa
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Visualization of Twitching Motility and Characterization of the Role of the PilG in Xylella fastidiosa

Article DOI: 10.3791/53816 • 08:44 min • April 8th, 2016
April 8th, 2016 •
1, 2
1Department of Plant Science, University of California, Davis, 2Agricultural Research Service, San Joaquin Valley Agricultural Sciences Center, United States Department of Agriculture

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Summary
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In this study, a nano-microfluidic flow chamber was employed to visualize and functionally characterize the twitching motility of Xylella fastidiosa, a bacterium that causes Pierce's disease in grapevine.

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Twitching Motility Bacteria Genes PilG Xylella Fastidiosa Nano Microfluidic Flow Chamber Time-lapse Image Recording System Phenotype Gene Function Observation Aggregation Migration Cells Populations Deletion Mutants Standardized Deletion Strategy PD2 Medium Agar Plates Incubate Gram-negative Bacterium Economically Important Diseases Plants Long-distance Intra-plant Movement Colonization Pathogenicity Type IV Pili Chemotaxis Regulator
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