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JoVE Journal
Immunology and Infection

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Visualization of Twitching Motility and Characterization of the Role of the PilG in Xylella fastidiosa
 

Visualization of Twitching Motility and Characterization of the Role of the PilG in Xylella fastidiosa

Article DOI: 10.3791/53816 08:44 min April 8th, 2016
April 8th, 2016

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Summary

In this study, a nano-microfluidic flow chamber was employed to visualize and functionally characterize the twitching motility of Xylella fastidiosa, a bacterium that causes Pierce's disease in grapevine.

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Twitching Motility Bacteria Genes PilG Xylella Fastidiosa Nano Microfluidic Flow Chamber Time-lapse Image Recording System Phenotype Gene Function Observation Aggregation Migration Cells Populations Deletion Mutants Standardized Deletion Strategy PD2 Medium Agar Plates Incubate Gram-negative Bacterium Economically Important Diseases Plants Long-distance Intra-plant Movement Colonization Pathogenicity Type IV Pili Chemotaxis Regulator
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