Visualization of Twitching Motility and Characterization of the Role of the <em>PilG</em> in <em>Xylella fastidiosa</em>

Xiangyang Shi; Hong Lin

doi:10.3791/53816

Journal

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Immunology and Infection

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Visualization of Twitching Motility and Characterization of the Role of the PilG in Xylella fastidiosa

Visualization of Twitching Motility and Characterization of the Role of the <em>PilG</em> in <em>Xylella fastidiosa</em>

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

Visualization of Twitching Motility and Characterization of the Role of the PilG in Xylella fastidiosa

Visualization of Twitching Motility and Characterization of the Role of the PilG in Xylella fastidiosa

10,688 Views

•

08:44 min

•

April 08, 2016

DOI:

10.3791/53816-v

DOI:

10.3791/53816-v

•

08:44 min

April 08, 2016

•

2 Views

Xiangyang Shi¹, Hong Lin²

¹Department of Plant Science,University of California, Davis, ²Agricultural Research Service, San Joaquin Valley Agricultural Sciences Center,United States Department of Agriculture

Transcript

The over goal of this protocol is to functionally characterize the genes responsbile for the twitching motility of bacteria using a nano microfluidic flow chamber integrated with a time-lapse image recording system.This method can help answer key questions about a bacteria phenotype and associated gene function, such as the twitching motility of bacteria.The main advantage of this technique is that it provides a special and a tempered observation for aggregation migration of individual cells and populations.Demonstrating this procedure will be Dr.Xiangyang Shi, a post-doc from my laboratory.Using a standardized deletion strategy, grow Temecula wild-type Xylella fastidiosa, grow pilG deletion mutants, and grow the complimentary strain all on PD2 medium agar plates.Incubate the bacteria at 28

Summary

In this study, a nano-microfluidic flow chamber was employed to visualize and functionally characterize the twitching motility of Xylella fastidiosa, a bacterium that causes Pierce's disease in grapevine.