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Generatie van ESC afgeleide Mouse epitheelcellen behulp van cellen ontdane Lung Steigers
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Generation of ESC-derived Mouse Airway Epithelial Cells Using Decellularized Lung Scaffolds
DOI:

12:31 min

May 05, 2016

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Chapters

  • 00:05Title
  • 00:47Scaffold Preparation
  • 03:57Thick Section Generation
  • 07:17Decontamination of Scaffold Sections and Recellularization Setup
  • 09:46Results: ESC-derived Mouse Airway Epithelial from Decellularized Lung Scaffolds
  • 11:52Conclusion

Summary

Automatic Translation

Dit protocol richt efficiënt muis embryonale stamcellen afgeleide definitieve endoderm aan de luchtwegen rijpen epitheelcellen. Deze differentiatie techniek maakt gebruik van 3-dimensionale cellen ontdane long scaffolds rechtstreekse long lijn specificatie, in een gedefinieerd serumvrij cultuurmedium setting.

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