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Generering av MGP-avledet Mouse Airway Epitelceller Bruke Decellularized Lung Stillas
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Chapters
- 00:05Title
- 00:47Scaffold Preparation
- 03:57Thick Section Generation
- 07:17Decontamination of Scaffold Sections and Recellularization Setup
- 09:46Results: ESC-derived Mouse Airway Epithelial from Decellularized Lung Scaffolds
- 11:52Conclusion
Denne protokollen dirigerer effektivt mus embryonale stamcelle-avledet definitive endoderm til modne luftveier epitelceller. Denne differensieringen teknikken bruker tre-dimensjonale decellularized lunge stillasene for å dirigere lunge avstamning spesifikasjon, i et definert, serumfritt kultur innstilling.
Tags
Decellularized Lung ScaffoldsEmbryonic Stem Cell DifferentiationAirway Epithelial Cells3D CultureLung DevelopmentCell Matrix ContractionsGrowth Factor SignalingSerum-free SettingRobust Airway Epithelial DifferentiationLimited Endoderm Lineage ContaminationRat LungsPerfusionTracheal CannulationDecellularization Solution
