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Bioengineering
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用脱细胞支架龙ESC衍生鼠气道上皮细胞的生成
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Generation of ESC-derived Mouse Airway Epithelial Cells Using Decellularized Lung Scaffolds
Please note that all translations are automatically generated.
Click here for the English version.
用脱细胞支架龙ESC衍生鼠气道上皮细胞的生成
DOI:
10.3791/54019-v
•
12:31 min
•
May 05, 2016
•
Sharareh Shojaie
,
Joyce Lee
,
Jinxia Wang
,
Cameron Ackerley
,
Martin Post
1
Department of Physiology and Experimental Medicine, Peter Gilgan Centre for Research and Learning
,
Hospital for Sick Children
Chapters
00:05
Title
00:47
Scaffold Preparation
03:57
Thick Section Generation
07:17
Decontamination of Scaffold Sections and Recellularization Setup
09:46
Results: ESC-derived Mouse Airway Epithelial from Decellularized Lung Scaffolds
11:52
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议有效地引导小鼠胚胎干细胞衍生定形内胚层成熟呼吸道上皮细胞。这种分化技术使用的3维脱细胞肺支架直接肺谱系说明书中,在一个确定的,无血清培养的设置。
Tags
Decellularized Lung Scaffolds
Embryonic Stem Cell Differentiation
Airway Epithelial Cells
3D Culture
Lung Development
Cell Matrix Contractions
Growth Factor Signaling
Serum-free Setting
Robust Airway Epithelial Differentiation
Limited Endoderm Lineage Contamination
Rat Lungs
Perfusion
Tracheal Cannulation
Decellularization Solution
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