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Die Messung der Kohlendioxidproduktion von Radiolabeled Substrate in<em> Drosophila melanogaster</em
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Biology
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JoVE Journal Biology
Measurement of Carbon Dioxide Production from Radiolabeled Substrates in Drosophila melanogaster
Please note that all translations are automatically generated. Click here for the English version.

Die Messung der Kohlendioxidproduktion von Radiolabeled Substrate in<em> Drosophila melanogaster</em

DOI:
10.3791/54045-v

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10:01 min

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June 27, 2016

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1Department of Pharmacology,University of Virginia

Chapters

  • 00:05Title
  • 00:48Preparation of Fly Food Containing Radiolabeled Metabolic Substrates
  • 02:16Feeding Radiolabeled Metabolic Substrates to Adult Fruit Flies
  • 04:03Preparation of the CO2-Collection Apparatus
  • 06:51Analysis of Results
  • 07:45Results
  • 09:05Conclusion

Summary

Automatic Translation

Automatic Translation

This paper describes a method for the measurement of fuel oxidation in Drosophila melanogaster in which trace amounts of specific radiolabeled metabolic substrates are fed to flies. The exhaled radiolabeled CO2 that is a produced from fuel oxidation is collected and measured.

Tags

Keywords: Carbon Dioxide ProductionRadiolabeled SubstratesDrosophila MelanogasterEnergy MetabolismFuel OxidationCarbon-14Fly Food VialCarbon Dioxide AnesthesiaFood Dye

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