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Detecting Anastasis In Vivo by CaspaseTracker Biosensor
JoVE Journal
Medicine
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JoVE Journal Medicine
Detecting Anastasis In Vivo by CaspaseTracker Biosensor
DOI:

20:16 min

February 01, 2018

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Chapters

  • 00:05Title
  • 04:50Preparing the Caspase Tracker Biosensor Flies
  • 09:29Transient Cell Death Induction
  • 11:10Fixation and Staining of Egg Chambers
  • 13:58Results: Reversal of Cell Death Process in Drosophila
  • 18:44Conclusion

Summary

Automatic Translation

Anastasis is technically challenging to detect in vivo because the cells that have reversed the cell death process can be morphologically indistinguishable from normal healthy cells. Here we describe protocols for detecting and tracking cells that undergo anastasis in live animals by using our newly developed in vivo CaspaseTracker biosensor system.

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