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用 CaspaseTracker 生物传感器检测 Anastasis体内
JoVE Journal
Medicine
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JoVE Journal Medicine
Detecting Anastasis In Vivo by CaspaseTracker Biosensor
DOI:

20:16 min

February 01, 2018

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Chapters

  • 00:05Title
  • 04:50Preparing the Caspase Tracker Biosensor Flies
  • 09:29Transient Cell Death Induction
  • 11:10Fixation and Staining of Egg Chambers
  • 13:58Results: Reversal of Cell Death Process in Drosophila
  • 18:44Conclusion

Summary

Automatic Translation

Anastasis 在技术上具有挑战性检测在体内, 因为逆转细胞死亡过程的细胞可以从形态上区分正常健康细胞。在这里, 我们描述了通过使用我们新近开发的体内CaspaseTracker 生物传感器系统来检测和跟踪在活体动物中 anastasis 的细胞的协议。

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