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Preparation of Homogeneous MALDI Samples for Quantitative Applications
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Biochemistry
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JoVE Journal Biochemistry
Preparation of Homogeneous MALDI Samples for Quantitative Applications

Preparation of Homogeneous MALDI Samples for Quantitative Applications

DOI:
10.3791/54409-v

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08:01 min

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October 28, 2016

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, , , , ,
1Genomic Research Center,Academia Sinica, 2Department of Chemistry,National Taiwan University, 3Department of Chemical Engineering and Biotechnology,National Taipei University of Technology

Chapters

  • 00:05Title
  • 01:09Preparation and Preconditioning
  • 02:05Sample Deposition and Drying
  • 03:55Mass Spectrometry Data Analysis
  • 05:39Results: MALDI Mass Spectrometry Analysis of Homogeneous Samples
  • 07:07Conclusion

Summary

Automatic Translation

Automatic Translation

A protocol for reducing spatial heterogeneities of ion signals in MALDI mass spectrometry by regulating substrate temperature during sample drying processes is demonstrated.

Tags

Keywords: MALDISample PreparationHomogeneous SamplesQuantitative ApplicationsDried-droplet MethodSpatial HeterogeneityDrying EnvironmentHydrodynamic FlowsTHAP Metric SystemAlpha-Cyano-4-hydroxycinnamic AcidDrying ChamberNitrogen FlowRelative HumidityTemperature ControlSample DepositionAnalyteCrystallization

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