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Capture og Frigivelse af levedygtige Cirkulerende tumorceller fra Blood
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Capture and Release of Viable Circulating Tumor Cells from Blood
DOI:

08:10 min

October 28, 2016

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Chapters

  • 00:05Title
  • 00:59Coating the Microfilter
  • 02:23Filtration Cassette Assembly
  • 03:14Filtration of the Blood Sample
  • 06:17Results: Effective Capture, Release, and Retrieval of Viable CTCs from Blood
  • 07:33Conclusion

Summary

Automatic Translation

En protokol til at udnytte en poly (N so-propylacrylamid) (PIPAAm) belagt mikrofilter til effektiv indfangning og termoresponsiv frigivelse af levedygtige cirkulerende tumorceller (CTC) præsenteres. Denne metode giver mulighed for indfangning af CTC fra patienternes blod og efterfølgende frigivelse af levedygtige CTC for nedstrøms off-chip kultur, analyser og karakterisering.

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