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Genetisk Manipulation af afgroedesygdomme<em> Ustilago maydis</em> At studere Fungal Biology og Plant Microbe Interactions
JoVE Journal
Genetics
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JoVE Journal Genetics
Genetic Manipulation of the Plant Pathogen Ustilago maydis to Study Fungal Biology and Plant Microbe Interactions
DOI:

11:42 min

September 30, 2016

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Chapters

  • 00:05Title
  • 00:46Protoplast Preparation
  • 04:08Transformation of U. maydis
  • 06:35Verification of Deletion Events: Preparing gDNA
  • 09:17Results: Deletion Mutant Experiment
  • 10:43Conclusion

Summary

Automatic Translation

Vi beskriver en robust generstatning strategi til genetisk manipulere smut svampen Ustilago maydis. Denne protokol forklarer, hvordan at generere deletionsmutanter at undersøge infektion fænotyper. Det kan udvides til at modificere gener i enhver ønsket måde, fx ved tilsætning af en sekvens, der koder et fluorescerende protein-tag.

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