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Journal / Genetics / Genetische Manipulation der Pflanze Pathogen
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Genetics

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JoVE Journal Genetics
Genetische Manipulation der Pflanze Pathogen
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Genetische Manipulation der Pflanze Pathogen

Article DOI: 10.3791/54522-v • 11:42 min • September 30th, 2016
September 30th, 2016 •
*1,2, *1, 3, 3, 1,2, 1,2,4
1Institute for Microbiology, Heinrich-Heine University Düsseldorf, 2Bioeconomy Science Center (BioSC), 3Department of Genetics, Institute of Applied Biosciences, Karlsruhe Institute of Technology, 4Cluster of Excellence in Plant Sciences (CEPLAS), Heinrich-Heine University Düsseldorf
* These authors contributed equally

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Wir beschreiben eine robuste Strategie Gen - Ersatz zu genetisch den Pilz Sauereien manipulieren Ustilago maydis. Dieses Protokoll wird erklärt, wie Deletionsmutanten zu erzeugen Infektion Phänotypen zu untersuchen. Es kann erweitert werden , um Gene in jeder gewünschten Weise zu modifizieren, beispielsweise durch eine Sequenz Hinzufügen eines fluoreszierenden Protein - Tag kodiert.

Tags

Genetik Ausgabe 115, Gentechnik Deletionsmutante reverse Genetik Verifikation Mutantenphänotyps homologe Rekombination Modell Brandpilz Pflanzenschädling Stamm
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