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Immunology and Infection
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CD4的制备<sup> +</sup> T细胞通过显微镜分析GD3和GD2神经节苷脂膜表达的
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Preparation of CD4
+
T Cells for Analysis of GD3 and GD2 Ganglioside Membrane Expression by Microscopy
Please note that all translations are automatically generated.
Click here for the English version.
CD4的制备<sup> +</sup> T细胞通过显微镜分析GD3和GD2神经节苷脂膜表达的
DOI:
10.3791/54569-v
•
10:00 min
•
November 08, 2016
•
Tania M. Villanueva-Cabello
,
Iván Martinez-Duncker
1
Instituto de Investigación en Ciencias Básicas y Aplicadas, Centro de Investigación en Dinámica Celular, Laboratorio de Glicobiología Humana y Diagnóstico Molecular
,
Universidad Autónoma del Estado de Morelos
Chapters
00:05
Title
01:00
Isolation and Activation of Human Naïve CD4
+
T Cells
04:22
Collection and Fixation of Resting and Activated Naïve CD4
+
T Cells
05:34
Staining, Mounting and Visualization by Confocal Microscopy
07:47
Results: Localization of GD3 and GD2 Gangliosides in Adhered Activated CD4
+
T Cells
09:13
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了在显微镜使用,以确定在静止神经节苷脂和激活的人幼稚CD4
+
T细胞的细胞膜表达和定位一个标准抗体染色方案。还使用<40,000个细胞,不需要额外的低输入RNA试剂描述的实时PCR实验。
Tags
CD4+ T Cells
GD3 Ganglioside
GD2 Ganglioside
Membrane Expression
Microscopy
Peripheral Blood Mononuclear Cells (PBMCs)
Negative Selection
Anti-CD3 Antibody
Anti-CD28 Antibody
Cell Stimulation
Cell Culture
Cell Preparation
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